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Sample report Illustrative data — not a real person's results Not a diagnosis

GutX Core Microbiome Profiling Report

GutX™ Core Microbiome Profiling Report

A genus-level description of the bacterial community in this illustrative stool sample.

Sample
SAMPLE-GUTX-000000
Report
sample-gutx-core-v1 · v1
Method
16S · V4 (515F/806R)
Generated
2026-08-22

This page renders from the same report contract a real report uses, with fixture data marked is_sample: true. Nothing here was produced from anyone's sample.

What this report contains

  • 3Reported
  • 2Partly reported
  • 2Withheld
  • 1Not applicable
  • This report describes which bacterial groups were detected and in what relative proportions. It does not identify disease, and nothing in it is a diagnosis.
  • Sequencing used the 16S rRNA gene V4 region (515F/806R primers), which resolves the bacterial community to genus. Species-level identity is not reported.
  • Fungi, viruses, archaea and bacterial gene function were not measured by this assay and are absent from this report by design, not by result.
  • Every number on this page is illustrative sample data created to demonstrate the report format.

Sample quality

Pass

Pass on the checks this pipeline performs. 3 checks in this list were not evaluated and are named below rather than counted as passing.

Quality control metrics, thresholds and results
Check Value Threshold applied Result
Reads passing quality filtering 61420 reads 10000 reads QC_Thresholds_Final · QC_01 Pass
Bases at Q30 or above 92.4 % 70 % QC_Thresholds_Final · QC_03 Pass
Reads assigned to a genus 94.1 % 80 % GutX Core report spec · QC_ASSIGN_01 Pass
Amplicon sequence variants recovered 412 ASVs No governed threshold Not evaluated
Negative-control contamination screen Not evaluated No governed threshold Not evaluated
Host DNA carry-over screen Not evaluated No governed threshold Not evaluated

Checks this pipeline does not perform

  • Negative-control contamination screen — this pipeline does not run one. Its absence is stated rather than reported as a pass.
  • Host DNA carry-over screen — not implemented for the 16S workflow.
  • ASV count is reported for transparency but is not gated against a governed threshold.

Community composition

Partly reported

Relative abundance is the share of assigned sequencing reads attributed to each group. It describes proportions within this sample only — it is not a count of organisms and cannot be compared directly against a different sequencing method.

Genus-level and phylum-level composition are released. Species-level composition is locked: the 16S V4 amplicon does not carry enough sequence variation to separate most species within a genus.

  • Bacillota (formerly Firmicutes)

    Measured

    48.2% Mid-range across the illustrative reference set

    Confidence 86%

    gutx-core-profile@1.0.0#phylum_relative_abundance

  • Bacteroidota (formerly Bacteroidetes)

    Measured

    31.7% Mid-range across the illustrative reference set

    Confidence 86%

    gutx-core-profile@1.0.0#phylum_relative_abundance

  • Actinomycetota (formerly Actinobacteria)

    Measured

    8.9% Mid-range across the illustrative reference set

    Confidence 82%

    gutx-core-profile@1.0.0#phylum_relative_abundance

  • Pseudomonadota (formerly Proteobacteria)

    Measured

    4.6% Mid-range across the illustrative reference set

    Confidence 80%

    gutx-core-profile@1.0.0#phylum_relative_abundance

  • Reads not assignable to a genus

    Measured

    5.9%

    • Unassigned reads are reported rather than redistributed. They are excluded from the proportions above, so those proportions describe the assigned fraction of the community.

    gutx-core-profile@1.0.0#unassigned_fraction

  • Species-level composition

    Measured

    Needs a different sequencing method TIER_REQUIRED

    Requires a new sample sequenced at a deeper tier. It cannot be unlocked on this result. Required sequencing tier: WGS.

    • The 16S V4 region amplified by the 515F/806R primer pair does not distinguish most species within a genus. Reporting species names from this data would be an inference presented as a measurement.
    • Species-level identity requires shotgun metagenomic sequencing — a different assay on a new sample, not an unlock on this one.
Phylum-level composition of the assigned fraction Measured
  • Bacillota 48.2 %
  • Bacteroidota 31.7 %
  • Actinomycetota 8.9 %
  • Pseudomonadota 4.6 %
  • Other assigned 6.6 %

Share of assigned reads (%)

Ten most abundant genera Measured
TaxonResolutionRelative abundance (%)
Bacteroides Genus 14.8
Faecalibacterium Genus 6.1
Blautia Genus 5.4
Prevotella Genus 4.9
Alistipes Genus 3.7
Bifidobacterium Genus 3.1
Roseburia Genus 2.4
Ruminococcus Genus 2.2
Akkermansia Genus 1.6
Eubacterium rectale group Genus group 1.2

What this section cannot tell you

  • Relative abundance shifts when any one group changes, so a proportion moving does not mean that organism's absolute quantity changed.
  • Stool sampling captures the luminal community; it is not a direct sample of the mucosal community.

Diversity

Reported

Diversity indices summarise how many distinct groups were detected and how evenly the reads are spread across them. They are descriptive summaries of this sample, and a higher or lower value is not by itself better or worse.

  • Shannon index (genus level)

    Derived

    3.42 Mid-range across the illustrative reference set

    Confidence 84%

    • Computed on the assigned fraction only. Unassigned reads are excluded rather than treated as a single group.

    gutx-core-profile@1.0.0#shannon_genus

  • Distinct genera detected

    Measured

    128 genera

    Confidence 79%

    • A raw count of detected genera, which rises with sequencing depth. It is not a depth-corrected richness estimate.

    gutx-core-profile@1.0.0#observed_genera

  • Pielou evenness

    Derived

    0.71 Mid-range across the illustrative reference set

    Confidence 79%

    • Derived from the observed genus count, so it inherits that count's dependence on sequencing depth.

    gutx-core-profile@1.0.0#pielou_evenness

Shannon index on the reported scale Derived

3.42

0 Shannon index (genus level) 5

Scale endpoints are the reporting range for this index, not a target.

What this section cannot tell you

  • Diversity indices are not comparable across sequencing methods, primer pairs or bioinformatics pipelines.

Short-chain fatty acid producer ecology

Reported

This panel sums the relative abundance of genera that the published literature associates with short-chain fatty acid production. It describes who is present, not what they are doing.

  • Combined relative abundance of SCFA-associated genera

    Derived

    18.4% Mid-range across the illustrative reference set

    Confidence 74%

    • This is a sum of taxon abundances, not a measurement of short-chain fatty acids. No metabolite was measured in this assay.
    • Membership of the guild is defined by a fixed, versioned taxon list. Organisms outside that list that also ferment are not counted.

    gutx-core-profile@1.0.0#scfa_guild_aggregate

  • Relative abundance of butyrate-associated genera

    Derived

    9.7% Mid-range across the illustrative reference set

    Confidence 72%

    • 16S data cannot confirm that these organisms are producing butyrate in this sample. Presence of a genus is not evidence of activity.

    gutx-core-profile@1.0.0#butyrate_guild_aggregate

Contributors to the SCFA-associated panel Measured
  • Faecalibacterium 6.1 %
  • Blautia 5.4 %
  • Roseburia 2.4 %
  • Ruminococcus 2.2 %
  • Eubacterium rectale group 1.2 %
  • Anaerostipes 0.6 %
  • Coprococcus 0.5 %

Relative abundance (%)

What this section cannot tell you

  • A guild aggregate is a reading aid. It compresses several independent taxon abundances into one figure and hides which organism moved.

Functional potential

Withheld

Needs a different sequencing method TIER_REQUIRED

Not available from 16S sequencing. Predicting gene content from a 16S marker gene produces an inference about what an average member of a genus might carry, not a measurement of what this community carries. GutX does not publish that inference as a result.

Requires a new sample sequenced at a deeper tier. It cannot be unlocked on this result. Required sequencing tier: WGS.

  • Metabolic pathway potential

    Inferred

    Not reported — needs a different sequencing method

    • Requires shotgun metagenomic sequencing (WGS) to observe gene content directly.
    • Even WGS reports genomic potential, not gene expression. Expression requires a metatranscriptomic assay.

What this section cannot tell you

  • This section needs a different sequencing method on a new sample. It cannot be added to this result after the fact.

Opportunist genus context

Partly reported

Several genera contain both commonly-carried members and members that are studied as opportunists. Detecting the genus does not identify which member is present, and detection at these proportions is a normal finding in sequencing data from people with no symptoms.

Genus-level detection is released. Species-level identification within these genera is locked, because 16S V4 cannot separate the species that carry different clinical meaning.

  • Escherichia-Shigella (genus group)

    Measured

    0.42% Low share of the assigned community

    Confidence 77%

    • Escherichia and Shigella are not separable at the 16S V4 region and are reported as a single group.

    gutx-core-profile@1.0.0#opportunist_genus_abundance

  • Enterococcus

    Measured

    0.28% Low share of the assigned community

    Confidence 77%

    gutx-core-profile@1.0.0#opportunist_genus_abundance

  • Klebsiella

    Measured

    0.11% Low share of the assigned community

    Confidence 73%

    gutx-core-profile@1.0.0#opportunist_genus_abundance

  • Species-level identification within these genera

    Measured

    Needs a different sequencing method TIER_REQUIRED

    Requires a new sample sequenced at a deeper tier. It cannot be unlocked on this result. Required sequencing tier: WGS.

    • Naming a species from 16S V4 data in these genera would be a guess. GutX withholds the name rather than printing one.
    • This assay is not a clinical pathogen test and must not be used to rule an infection in or out.
Detection summary Measured
TaxonRelative abundance (%)Resolution reached
Escherichia-Shigella 0.42 Genus group
Enterococcus 0.28 Genus
Klebsiella 0.11 Genus

What this section cannot tell you

  • This panel is descriptive context from a research-grade assay. It is not a diagnostic microbiology culture and does not detect toxins, resistance genes or viability.

ATLAS reference positioning

Withheld

Not included in this report ENTITLEMENT_REQUIRED

Positioning this sample against a governed ATLAS reference cohort is part of ATLAS Intelligence, which is not included in a Core Microbiome Profiling Report.

Included with a report tier that covers it.

  • Position against a governed reference cohort

    Reference comparison

    Not reported — not included in this report

    • No ATLAS reference artifact was consumed by this report. Nothing on this page is a comparison against other people.

Change over time

Not applicable

Not applicable to this report

This is the first sample on this account, so there is nothing to compare it against. This section appears once a second comparable sample has been processed.

Method and limits

Reported

What this assay measured, and what it did not. Everything absent from this list is absent from the report by design.

Explanatory

  • What was sequenced

    The V4 hypervariable region of the bacterial 16S rRNA gene, amplified with the 515F/806R primer pair and classified against SILVA 138.1.

  • Resolution reached

    Genus. Species-level identity is not reported from this assay, because the V4 region does not carry enough variation to support it.

  • What was not measured

    Fungi, viruses, archaea, parasites, human cells, metabolites, gene content and gene expression. None of these were assayed.

  • What this report is not

    It is not a diagnostic test, not a screen for any disease, and not a basis for starting, stopping or changing any treatment. Discuss any health concern with a qualified clinician.

How to read the method labels

  • Measured Directly observed in this sample, within the limits of the assay.
  • Derived Calculated from measured values using a fixed, versioned formula.
  • Inferred A prediction, not an observation. Nothing here was measured directly.
  • Reference comparison Positioned against a governed reference cohort.
  • Explanatory Explanatory text. Carries no data from this sample.

Limitations of this report

  • Every value in this report is illustrative sample data. It did not come from a person and describes no one's sample.
  • This is a descriptive profiling report. It does not diagnose, screen for, treat or rule out any condition.
  • Sequencing covered the 16S rRNA V4 region only (515F/806R). Results resolve to genus; species are not reported.
  • Fungi, viruses, archaea, parasites, metabolites and gene function were not measured.
  • No ATLAS reference cohort was consulted. Nothing here positions this sample against other people.
  • A negative-control contamination screen and a host-DNA screen are not part of this pipeline. Those checks are reported as not evaluated, never as passed.
  • Cryptographic hashes of the input artifacts are not carried on a sample report. A real report carries a sha256 for every input consumed.

Provenance

Every version that contributed to this report. Fields with nothing behind them are shown as gaps rather than filled with a plausible default.

Report identity

Report ID
sample-gutx-core-v1
Report version
1
Schema version
1.0.0
Sample ID
SAMPLE-GUTX-000000
Run ID
SAMPLE-RUN-GUTX-000000
Sequencing tier
16S
Generated
2026-08-22T09:00:00.000Z

Pipeline and reference data

Pipeline
gutx-16s-core@0.9.0
Feature schema
vams-feature-schema@1.2.0
Report specification
gutx-core-profile@1.0.0
Taxonomy database
SILVA
Taxonomy database version
138.1 (99% identity, 515F/806R region-trimmed)
Classifier
QIIME 2 2024.5 · q2-feature-classifier vsearch consensus
Classifier SHA-256
Not carried
Functional method
Not carried
Functional method version
Not carried

ATLAS reference

No ATLAS reference artifact was consumed. Nothing in this report compares this sample against a reference cohort or against other people.

Score definition version
Not applicable
Condition taxonomy version
Not applicable
Reference artifact set
Not applicable
Reference cohort version
Not applicable

Input artifact hashes

Not carried on a sample report. A report generated from a real sample carries a SHA-256 for every input artifact consumed.

All provenance fields the contract requires for an auditable report are present.

Sample report · every value on this page is illustrative · not a diagnosis and not medical advice